Immunofluorescence:Article Title: New insights into intranuclear inclusions in thyroid carcinoma: Association with autophagy and with BRAF V600E mutation
Article Snippet: .. Double-labeling immunofluorescence microscopy: BRAFV600E/LC3B, BRAFV600E/p62 and BRAFV600E/ubiquitin Double-labeling immunofluorescence microscopy for BRAFV600E and LC3B A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV600E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min., 2x LC3B Cell Signaling #3868 Mono Rabbit D11 1:100 room temperature, 60min.,2x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x LC3B Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature 60min., 2x A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV6000E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min. .. 2x p62 Enzo BML PW9860 Poly Rabbit 1:250 room temperature, 60min.,1x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x p62 Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature, 60min.,1x A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV600E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min.,2x Ubiquitin Dako Z0458 Poly Rabbit 1:500 room temperature, 60min.,1x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x Ubiquitin Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature 60min.,1x
Microscopy:Article Title: New insights into intranuclear inclusions in thyroid carcinoma: Association with autophagy and with BRAF V600E mutation
Article Snippet: .. Double-labeling immunofluorescence microscopy: BRAFV600E/LC3B, BRAFV600E/p62 and BRAFV600E/ubiquitin Double-labeling immunofluorescence microscopy for BRAFV600E and LC3B A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV600E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min., 2x LC3B Cell Signaling #3868 Mono Rabbit D11 1:100 room temperature, 60min.,2x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x LC3B Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature 60min., 2x A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV6000E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min. .. 2x p62 Enzo BML PW9860 Poly Rabbit 1:250 room temperature, 60min.,1x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x p62 Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature, 60min.,1x A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV600E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min.,2x Ubiquitin Dako Z0458 Poly Rabbit 1:500 room temperature, 60min.,1x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x Ubiquitin Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature 60min.,1x
Incubation:Article Title: New insights into intranuclear inclusions in thyroid carcinoma: Association with autophagy and with BRAF V600E mutation
Article Snippet: .. Double-labeling immunofluorescence microscopy: BRAFV600E/LC3B, BRAFV600E/p62 and BRAFV600E/ubiquitin Double-labeling immunofluorescence microscopy for BRAFV600E and LC3B A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV600E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min., 2x LC3B Cell Signaling #3868 Mono Rabbit D11 1:100 room temperature, 60min.,2x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x LC3B Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature 60min., 2x A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV6000E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min. .. 2x p62 Enzo BML PW9860 Poly Rabbit 1:250 room temperature, 60min.,1x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x p62 Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature, 60min.,1x A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV600E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min.,2x Ubiquitin Dako Z0458 Poly Rabbit 1:500 room temperature, 60min.,1x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x Ubiquitin Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature 60min.,1x
Labeling:Article Title: New insights into intranuclear inclusions in thyroid carcinoma: Association with autophagy and with BRAF V600E mutation
Article Snippet: .. Double-labeling immunofluorescence microscopy: BRAFV600E/LC3B, BRAFV600E/p62 and BRAFV600E/ubiquitin Double-labeling immunofluorescence microscopy for BRAFV600E and LC3B A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV600E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min., 2x LC3B Cell Signaling #3868 Mono Rabbit D11 1:100 room temperature, 60min.,2x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x LC3B Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature 60min., 2x A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV6000E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min. .. 2x p62 Enzo BML PW9860 Poly Rabbit 1:250 room temperature, 60min.,1x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x p62 Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature, 60min.,1x A: Primary Antibodies anti- manufacturer order number host dilution Incubation conditions BRAFV600E abcam ab228461 Mono Mouse IgG2a VE1 1:100 room temperature, 60min.,2x Ubiquitin Dako Z0458 Poly Rabbit 1:500 room temperature, 60min.,1x B: Fluorochrome labeling BRAFV600E Vecta Fluor Excel Vector DK-2594 DyLight 594 ready to use, room temperature, 30min., 2x Ubiquitin Donkey Anti Rabbit Invitrogen A21206 Alexa Fluor 488 1:100, room temperature 60min.,1x
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